A bilirubin oxidase produced by Penicillium sp. strain LAM 91-89 was purified and partially characterized. The enzyme was purified about 70 folds from culture broth by ethanol precipitation, first and second Sephadex G-200 column chromatography with overall yield of 12%. The molecular weight of the enzyme was estimated to be 53,000 dalton by SDS-PAGE. The optimum pH and temperature was 8.5 and 40¡É, respectively. The enzyme was stable in the pH range 6¡10 and below 40¡É. Activity of the enzyme was increased by Che addition of Mg^(2+) but was gretly inhibited by Ag^(2+), Hg^(2+), Mn^(2+), Pb^(2+), iodoacetate, p-chloromercurobenzoic acid and sodium dodecyl sulfate. Among various substrates, bilirubin was favorably reacted and K_m value for bilirubin was 6.67 ¥ìmole.
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